1. ANALIZA ELECTROFORETICĂ A PROTEINELOR LACRIMALE - METODĂ DE DIAGNOSTIC A SINDROMULUI SJOGREN SECUNDAR

VIORICA MADALINA COJOCARU, COZIANA CIURTIN*, ELENA PUCHIANU**, FELICIA ANTOHE**
SPITALUL CLINIC DE URGENTE OFTALMOLOGICE, BUCURESTI
*SPITALUL CLINIC "DR. I. CANTACUZINO", BUCURESTI
**INSTITUTUL DE BIOLOGIE SI PATOLOGIE CELULARA "N. SIMIONESCU", BUCURESTI

REZUMAT
Scop: Aceasta lucrare si-a propus analiza si compararea aspectelor electroforetice ale proteinelor din lacrimile recoltate de la subiecti sanatosi si de la pacienti cu boli autoimune asociate cu o incidenta crescuta a sindromului Sjogren secundar (SSs).

Material si Metoda: Lacrimile au fost recoltate folosind metoda Schirmer I. Separarea electroforetica s-a realizat pe un gel sodium dodecyl sulfate-poliacrylamidic (SDS-PAGE) iar colorarea gelului s-a realizat cu argint si/sau Coomassie blue.
Rezultate: Principalele componente proteice separate din lacrimi au fost: lactoferina, albumina, lipocalina si lizozimul si au fost identificate folosind markeri de greutate moleculara.

Concluzii: Analiza electroforetica a secretiei lacrimale reprezinta o metoda rapida si simpla care poate furniza informatii precoce despre o posibila afectare a glandei lacrimale in cadrul bolilor autoimune.

CUVINTE CHEIE:

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ABSTRACT
Purpose: We analysed and compared electrophoretic tear proteins patterns of healthy subjects and patients with different autoimmune diseases associated with secondary Sjogren's syndrome.
Materials and Methods: Tears were collected using the Schirmer's method. Proteins were separated by sodium-dodecyl sulfate poliacrylamide gel electrophoresis. The lanes were stained by Coomassie blue and/or silver.
Results: Lactoferrin, albumin, lipocalin and lysozyme were found to be the main components being identified using molecular weight markers.
Conclusions: Electrophoretic analysis of tear proteins patterns is a fast, reproducible and simple method which provides information about the possibility of lacrimal gland involvement in autoimmmune diseases.

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